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Research

Muscle stem cell isolation and in vitro culture for meat production: A methodological review

Cultured muscle tissue-based protein products, also known as cultured meat, are produced through in vitro myogenesis involving muscle stem cell culture and differentiation, and mature muscle cell processing for flavor and texture. This review focuses on the in vitro myogenesis for cultured meat production. The muscle stem cell-based in vitro muscle tissue production consists of a sequential process: (1) muscle sampling for stem cell collection, (2) muscle tissue dissociation and muscle stem cell isolation, (3) primary cell culture, (4) upscaled cell culture, (5) muscle differentiation and maturation, and (6) muscle tissue harvest. Although muscle stem cell research is a well-established field, the majority of these steps remain to be underoptimized to enable the in vitro creation of edible muscle-derived meat products. The profound understanding of the process would help not only cultured meat production but also business sectors that have been seeking new biomaterials for the food industry. In this review, we discuss comprehensively and in detail each step of cutting-edge methods for cultured meat production. This would be meaningful for both academia and industry to prepare for the new era of cellular agriculture.

Source: Muscle stem cell isolation and in vitro culture for meat production: A methodological review – PubMed

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Compagnies and key players

Will Cultured Bacon Be Delicious? A Dutch Startup Is Developing the First Lab-Grown Pork

But here’s where Meatable has an edge. While many of the other 40-odd companies working in the cultured meat space use fetal bovine serum or Chinese hamster ovary cells to stimulate cell division and production, Meatable has licensed a technology dubbed OPTi-OX, which involves engineering induced pluripotent stem cells for specific cell types then ‘reprogramming’ them to adult stem cells. The process yields consistent, homogeneous, rapid cell batches—in other words, a full steak in a matter of weeks.

Source: Will Cultured Bacon Be Delicious? A Dutch Startup Is Developing the First Lab-Grown Pork – Clean Meat News Australia